Fig. 2

Confirmation of the optimal concentrations of rapamycin and MHY1487 for Caco-2 cells. (A) Effect of different concentrations of rapamycin on the viability of Caco-2 cells. (B) Effect of different concentrations of rapamycin on the expression of p-mTOR in Caco-2 cells. (C) Effect of different concentrations of MHY1487 on the viability of Caco-2 cells. (D) Effect of different concentrations of MHY1487 on the expression of p-mTOR in Caco-2 cells. Optimal concentrations for rapamycin and MHY1487 were 10 nM and 100 nM, respectively. ^*P < 0.05; ^**P < 0.01; ^****P < 0.0001. vs. 0 nM