Fig. 3

Effects of HIF-1α activators and inhibitors on the TEER (A), FD-4 concentration (B), and the expression of HIF-1α (C, D) and TJ-related proteins (C, E-G) in the Caco-2 cell epithelial model after LPS stimulation. LPS-induced damage to Caco-2 cell epithelial model showed the decrease of TEER value, the increase of FD4 concentration and down-regulation of TJ-related proteins. DMOG, the HIF-1α activator, alleviated the LPS-induced damage to Caco-2 cell epithelial model, increased TEER value, decreased FD4 concentration and up-regulated the expression of TJ-related proteins. BAY 87-2243, a HIF-1α inhibitor, had the opposite effect. LPS up-regulated HIF-1α expression, DMOG further up-regulated HIF-1α expression, while BAY 87-2243 had the opposite effect

Con: control group, LPS: LPS group, L + D: LPS + DMOG group, L + B: LPS + BAY 87-2243 group. *P < 0. 05, **P < 0. 01 vs. Con group; #P < 0.05, #P < 0.01 vs. LPS group