TY - JOUR AU - Litman, Pninit AU - Amieva, Manuel Ricardo AU - Furthmayr, Heinz PY - 2000 DA - 2000/11/01 TI - Imaging of Dynamic Changes of the Actin Cytoskeleton in Microextensions of Live NIH3T3 Cells with a GFP Fusion of the F-Actin Binding Domain of Moesin JO - BMC Cell Biology SP - 1 VL - 1 IS - 1 AB - The cell surface undergoes continuous change during cell movement. This is characterized by transient protrusion and partial or complete retraction of microspikes, filopodia, and lamellipodia. This requires a dynamic actin cytoskeleton, moesin, components of Rho-mediated signal pathways, rearrangement of membrane constituents and the formation of focal adhesion sites. While the immunofluorescence distribution of endogenous moesin is that of a membrane-bound molecule with marked enhancement in some but not all microextensions, the C-terminal fragment of moesin co-distributes with filamentous actin consistent with its actin-binding activity. By taking advantage of this property we studied the spontaneous protrusive activity of live NIH3T3 cells, expressing a fusion of GFP and the C-terminal domain of moesin. SN - 1471-2121 UR - https://doi.org/10.1186/1471-2121-1-1 DO - 10.1186/1471-2121-1-1 ID - Litman2000 ER -