Figure 5

DIC and fluorescence time-lapse imaging of microextensions in live C-moesin-GFP transfected cells. C-moesin-GFP fluorescence is seen to withdraw from and to re-enter an attached microextension (arrows). Note that the fluorescence intensities of C-moesin-GFP in two closely spaced microextensions, and hence their actin filament contents, are independent of each other. This is illustrated on the right side of the figure by showing intensity distributions at three different points (indicated by lines B (for Base), M (for middle) and T (for Tip) in the four panel at the very bottom) along the two filopodia. See also three related movies