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Figure 5 | BMC Cell Biology

Figure 5

From: C/EBPBeta and Elk-1 synergistically transactivate the c-fos serum response element

Figure 5

The C-terminal domain of C/EBPβ interacts with Elk-1. Radiolabeled Elk-1 was prepared by in vitro transcription and translation of CMV-Elk-1 expression construct and then incubated with equivalent amounts of GST, GST-p35-C/EBPβ, or GST-p20-C/EBPβ protein immobilized on glutathione-Sepharose. Proteins eluted from the GST-p20-C/EBPβ beads (lane 2), the GST-p35-C/EBPβ beads (lane 3) or GST beads (lanes 4) were resolved on an SDS-10% polyacrylamide gel and detected by autoradiography. Lane 1 contains one-half of the amount of the radiolabeled proteins initially mixed with the beads.

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