Figure 4From: Different conformations of nascent polypeptides during translocation across the ER membraneDetermination of the "minimal glycosylation distance" (MGD). (A) The MGD is defined as the minimum number of residues required to bridge the distance between the lumenal end of a transmembrane segment (white) and the active site of oligosaccharyl transferase (Y denotes a glycosylated site, Φ a non-glycosylated site). (B) MGD measurements for constructs with the indicated model segments. Half-maximal glycosylation (40%) is indicated by the horizontal line. The MGD value is counted from the first polar residue before the transmembrane segment (Gln) up to the acceptor Asn residue as in [14].Back to article page