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Figure 4 | BMC Cell Biology

Figure 4

From: Different conformations of nascent polypeptides during translocation across the ER membrane

Figure 4

Determination of the "minimal glycosylation distance" (MGD). (A) The MGD is defined as the minimum number of residues required to bridge the distance between the lumenal end of a transmembrane segment (white) and the active site of oligosaccharyl transferase (Y denotes a glycosylated site, Φ a non-glycosylated site). (B) MGD measurements for constructs with the indicated model segments. Half-maximal glycosylation (40%) is indicated by the horizontal line. The MGD value is counted from the first polar residue before the transmembrane segment (Gln) up to the acceptor Asn residue as in [14].

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