Figure 7

Integrin α3 and LN332 contribute to insulin-induced accelerating healing. (A) Representative images of excision wounds performed as in Figure 1. After wounding, some wounds were treated with 5 μl LN332 Ab (P3H9-2, 1 mg/ml) for 5 min, followed by 0.03 U insulin/30 μl PBS; other wounds were treated with 0.03 U insulin alone. Treatment was administered every two days until all wounds were closed. (B) Wound areas were quantified every two days and expressed as percentage of the area of the control (n = 3). Statistics are shown as comparisons between the LN332 Ab + insulin and insulin treatment. *P < 0.05. (C) Excision wounds were performed as above, except that wounds were treated with 5 μl of the anti-integrin α3 Ab (P1B5, 1 mg/ml) rather than the LN332 Ab. Function-inhibiting anti-integrin α3 Ab inhibited insulin-induced wound healing at early stages. (D) Wound areas were quantified as in (B). Statistics are shown as comparisons between the anti-integrin α3 Ab + insulin and insulin treatment. *P < 0.05. (n = 3).