Figure 2
From: Loss of Dictyostelium HSPC300 causes a scar-like phenotype and loss of SCAR protein
HSPC300 is needed for efficient cell motility. (A) Under-agar chemotaxis assay. Vegetative cells were allowed to migrate up a gradient of folate (arrow) under a layer of agar. Scale bar represents 20 μm. (B) Migration speed. Time-lapse movies made under conditions similar to (A) of 10 wild type, IR48 scar null, IR55 hspc300 null and hspc300 null expressing Myc-HSPC300 or untagged control cells were analyzed and a mean speed calculated. Data shown here are the means and standard errors of three independent experiments. Error bars represent one standard error. hspc300 null cells are significantly slower than wild type (P < 0.01) and are comparable to scar null cells. Expression of Myc-HSPC300 or an untagged control rescues the motility of hspc300 null cells back to wild type.