Figure 1

Tight junction barrier function assayed in H413 clone-1 epithelial cell monolayers treated with anti-CD24 peptide antibody. Antibody to CD24 reduces passage of fluorochrome-labelled dextran across H413 epithelial cell monolayers. Due to passage of dextran into the lower compartment reaching equilibrium after the 7^th hour, the rate of transfer was calculated over the first 5 hours. Regression analysis representative of three independent experiments for time points from triplicate cultures (showing mean values ± s.e.m.). Cultures were permeable for the translocation of low molecular weight dextran (Alexa Fluor 647 10 kDa, ** refers to P < 0.01, paired t-test), but were impermeable to high molecular weight dextran tetramethylrhodamine (TMR, 2 MDa).