Figure 3From: CD24 regulated gene expression and distribution of tight junction proteins is associated with altered barrier function in oral epithelial monolayersWestern blot analysis for representative tight junction proteins extracted from H413 clone-1 cell monolayers. Data shown are representative of three independent experiments. Protein loading controls were confirmed by β-actin stain. (A) Showing increased expression of tight junction proteins ZO-1 (210 kDa), ZO-2 (160 kDa) and occludin (65 kDa) in cultures treated with anti-CD24 peptide antibody compared to H413 monolayers treated with isotype IgG1 (5 μg/ml) control antibody. (B) Bar graph confirmed increased expression of ZO-1, ZO-2 and occludin in anti-CD24 antibody treated sample compared to isotype control antibody by paired t-test (* Refers to P < 0.05). (C) Showing multiple bands in cultures treated with anti-CD24 peptide antibody for claudin-7 compared to H413 monolayers treated with isotype IgG1 (5 μg/ml) control antibody (see explanation: Results and discussion). Specific bands were not detected for claudin-3 or par-6 in either isotype control or anti-CD24 antibody-challenged culture extracts.Back to article page