Western blot analysis for representative tight junction proteins extracted from H413 clone-1 cell monolayers. Data shown are representative of three independent experiments. Protein loading controls were confirmed by β-actin stain. (A) Showing increased expression of tight junction proteins ZO-1 (210 kDa), ZO-2 (160 kDa) and occludin (65 kDa) in cultures treated with anti-CD24 peptide antibody compared to H413 monolayers treated with isotype IgG1 (5 μg/ml) control antibody. (B) Bar graph confirmed increased expression of ZO-1, ZO-2 and occludin in anti-CD24 antibody treated sample compared to isotype control antibody by paired t-test (* Refers to P < 0.05). (C) Showing multiple bands in cultures treated with anti-CD24 peptide antibody for claudin-7 compared to H413 monolayers treated with isotype IgG1 (5 μg/ml) control antibody (see explanation: Results and discussion). Specific bands were not detected for claudin-3 or par-6 in either isotype control or anti-CD24 antibody-challenged culture extracts.