Figure 8

Deletion mutations at the ZM region disrupt ALP107-273 localization. The percentages of cells with YFP localization at stress fibres. YFP was fused to ALP107-273 without any modifications (WT) or with deletions or mutations (deletions and mutations shown in graph). Note that four deletion mutants and the YS197-198FA point mutation (YS-FA) abrogated localization. In cells expressing YFP alone, < 1% were scored positive.