Figure 4
From: Centrosome proteins form an insoluble perinuclear matrix during muscle cell differentiation
The centrosome protein PCM-1 localizes to dense structures on the cytoplasmic site of the nuclear envelope. (A) Deconvolved image of a nucleus from a differentiated H-2Kb-tsA58 cell, expressing GFP-lamin A (green), and stained for PCM-1 (red) and DNA (blue). (B) Nucleus from a differentiated H-2Kb-tsA58 cell, stained for PCM-1 (red), and for nuclear pore complex proteins (NPC, green). Selected areas of the nuclei in (A) and (B) are shown enlarged on the right. (C) Immuno-electron microscopy of cryosections of differentiated C2C12 cells. Different views of cross-sections of the nucleus are shown. PCM-1 is labelled with antibody and protein A, coupled to 10 nm gold. White arrows indicate the outline of a layer of electron-dense material at the outer nuclear surface. Bars in (B) and (C), 1 μm.