Figure 3

Both CSN2 and CSN5 knockdowns result in reduced cell growth. K562 cells were transiently co-transfected with HKK plasmid together with either shVC, shCSN2 or shCSN5 plasmid. HKK positive cells were sorted 24 hours post-transfection and cells re-cultured. (A) Cell counts were taken daily and the cumulative growth calculated. The cumulative growth of shCSN2 and shCSN5 cells is shown relative to shVC. The insert graph has a different Y scale to highlight the differences between shCSN2 and shCSN5 cumulative growth profiles. Data shown are the mean ± s.e.m. of n = 3. * indicates a significant difference to shVC cell growth with a p value of less than 0.05. (B) Thymidine incorporation in shCSN2 cells relative to shVC cells was measured day 3, 5 and 7 post transfection. Data shown are the mean ± s.e.m. of n = 3. (C) Thymidine incorporation in shCSN5 cells relative to shVC cells was measured day 3 and 4 post transfection. Data shown are the mean ± s.e.m. of n = 3. * indicates significance with a p value of less than 0.05.