Figure 1

Transfection with pSIREN Puro, pBABE Puro or pLKO.1 Puro vectors causes PML aggregation in multiple cell types. (A) U2OS cells on glass coverslips were transiently transfected with empty vectors using Fugene transfection reagent and immunostained for PML (red) 48 h after transfection. PML antibodies were detected using a rhodamine-x conjugated goat anti mouse IgG. Cell nuclei were counterstained with DAPI (blue). Images were captured at 100× magnification. (B) MCF-7 and HELA cells on glass coverslips were transiently transfected with pCMV Neo or pLKO.1 PURO empty vector using Fugene transfection reagent and immunostained for PML (red) 48 h after transfection. PML antibodies were detected using a rhodamine-x conjugated goat anti mouse IgG. Cell nuclei were counterstained with DAPI (blue). Images were captured at 100× magnification.