Figure 4

Immunocytochemistry showing the polarity of tRPE cells. A) Bestrophin immunocytochemistry of tRPE cells grown on BCE-ECM using conditioned media. The green photograph was taken through the FITC filter cube showing the localization of bestrophin and the photograph was overlayed with the blue photograph taken through the DAPI filter cube showing the nuclear counterstain Hoechst 33258; B) Zonula Occludens-1 (ZO-1) immunocytochemistry of tRPE cells grown on BCE-ECM using conditioned media. The green photograph was taken through the FITC filter cube showing the localization of ZO-1 around the periphery of the cells and the photograph was overlayed with a blue photograph taken through the DAPI filter cube showing the nuclear counterstain Hoechst 33258.