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Figure 5 | BMC Cell Biology

Figure 5

From: Isolation and characterization of a spontaneously immortalized bovine retinal pigmented epithelial cell line

Figure 5

Immunocytochemistry of tRPE cells expressing cytoskeletal proteins. A) Vimentin immunocytochemistry of tRPE cells grown on BCE-ECM using conditioned media. The red photograph was taken through the TRITC filter cube showing the finger-like projections of vimentin, which was overlayed with a blue photograph taken through the DAPI filter cube showing the nuclear counterstain Hoechst 33258. B) Pan-cytokeratin immunocytochemistry of tRPE cells grown on BCE-ECM using conditioned media. The green photograph was taken through the FITC filter cube showing the localization of the cytokeratin, and overlayed with a blue photograph taken through the DAPI filter cube showing the nuclear counterstain Hoechst 33258.

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