Figure 3
Effect of GST-tagged profilin isoforms on β-actin polymerization kinetics. 5 μM actin and 15 μM each of GST-PFN1, GST-PFN3, and GST-PFN4 were pre-incubated prior to induction of actin polymerization by addition of KCl and MgCl2. Time courses of actin alone (red) or in the presence of GST-PFN1 (green), GST-PFN3 (blue) or GST-PFN4 (orange) are shown. PFN1 markedly delayed actin polymerization, PFN3 influenced polymerization kinetics to a lower extent, and PFN4 had no obvious effect.