Figure 2
From: Incomplete posttranslational prohormone modifications in hyperactive neuroendocrine cells
Dynamics of 18 K and 18 K* POMC biosynthesis in hyperactive Xenopus melanotrope cells. Neurointermediate lobes from black-adapted Xenopus laevis were pulse labelled with [35S]methionine/cysteine for ten minutes and chased for the indicated time periods. Aliquots of the cell lysates (10%) and the incubation media (20%) were analysed by 15% SDS-PAGE and autoradiography.