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Figure 3 | BMC Cell Biology

Figure 3

From: Protein kinase C activation disrupts epithelial apical junctions via ROCK-II dependent stimulation of actomyosin contractility

Figure 3

Classical PKC isoenzymes mediate OI-V-induced disruption of epithelial apical junctions. HPAF-II cells were treated for 5 h with either OI-V alone (1 μM) or in a combination with pharmacological inhibitors of different subfamilies of PKC isoenzymes. The barrier properties of cell monolayers were determined by TEER measurement (A), and the integrity of AJ and TJ was determined by immunofluorescence labeling for E-cadherin and occludin respectively (B). A dual inhibitor of classical and novel PKC, GF-109203X (10 μM), as well as a selective inhibitor of classical PKC, Gö 6976 (5 μM) significantly attenuate octylindiolactam-induced disruption of paracellular barrier and TJ/AJ disassembly, whereas inhibitor of a novel PKC, rottlerin (10 μM), has no effects. Bar, 20 μm.

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