Figure 4

Junctional disassembly in PKC-activated pancreatic epithelial cells is accompanied by reorganization of the apical actomyosin cytoskeleton. Confluent HPAF-II cell monolayers were exposed for 3 h to either vehicle or OI-V (1 μM) followed by fixation and dual fluorescence labeling for occludin (red) with either F-actin or NM IIA heavy chain (green). OI-V induces cell rounding and accumulation of F-actin and NM IIA at the lateral plasma membrane in the areas of disassembling TJ (arrows). Bar, 20 μm.