Figure 9From: Protein kinase C activation disrupts epithelial apical junctions via ROCK-II dependent stimulation of actomyosin contractilityInhibition of MLCK, calmodulin, and ERK1/2 does not affect OI-V-induced disassembly of epithelial apical junctions. HPAF-II cells were treated for 5 h with either OI-V alone (1 μM) or in a combination with pharmacological inhibitors of MLCK (ML-7 (20 μM)), calmodulin (W-7 (100 μM)), and ERK1/2 (U 1026 (5 μM)). The integrity of AJ and TJ was determined by immunofluorescence labeling for E-cadherin and occludin respectively. Neither inhibitor prevents OI-V-induced disassembly of AJ and TJ. Bar, 20 μm.Back to article page