Table 1 Primers for RT-PCR and real-time RT-PCR
Target gene | Forward sequence (5'-3') | Reverse sequence (5'-3') | AS (bp) | AT (°C) | GA |
|---|---|---|---|---|---|
TLR3 | GGTGGTCCCGTTAATTTCCT | CCCGAAAACATCCTTCTCAA | 240 | 56 | NM_126166 |
TLR7 | TGGAAATTTTGGACCTCAGC | TTGCAAAGAAAGCGATTGTG | 262 | 52 | NM_133211 |
TLR9 | TGCAGGAGCTGAACATGAAC | TAGAAGCAGGGGTGCTCAGT | 297 | 58 | NM_031178 |
β-actin | GCTACAGCTTCACCACCACAG | GGTCTTTACGGATGTCAACGTC | 288 | 60 | NM_007393 |