Time-lapse phase contrast micrographs showing G. obscuriglobus budding cell division. Bud initiates from the polar end of the mother cell, grows until it reaches the same size as the mother cell and finally separates from the mother cell. The full budding process takes over 10 hours and approximately 12 hours. Arrowhead indicates the bud at the initiation (frame 1) and after separation (frame 25). Unit of the time in each frame is minutes. Bar – 1 μm.