Transmission electron micrographs of high-pressure frozen, cryosubstituted and Epon-embedded G. obscuriglobus budding cells. Each micrograph represents a different budding stage. The bud initiates from the polar end of the mother cell containing a nucleoid (N) surrounded by a nucleoid envelope (NE) (A) and the bud increases in size without the presence of either the nucleoid or the nucleoid envelope (B). As the bud develops, the nucleoid is translocated into the bud and the nucleoid envelope starts to develop (C, D and E) until forming a fully enclosed nuclear body (F). The bud continues developing until reaching a similar size to that of the mother cell (G), and the matured bud finally separates from the mother cell (H).Bar 500 nm (images A and E), 1 μm (images B, C, D, F, G and H).