Sample-to-sample comparison of relative signal intensities of four major N-glycan signals. Sample-to-sample comparison of relative signal intensities of four major N-glycan signals in the whole dataset of four hESC lines (FES 21, FES 22, FES 29, and FES 30), embryoid bodies derived from them (EB), stage 3 differentiated cells (st.3), and two human fibroblast samples from the same cell line that was used as feeder cells in the propagation of hESC. A. Glycan signal S1H5N4F2 is characteristic to hESC. B. Glycan signal S1H5N5F1 is characteristic to differentiated cells. C. and D. The major N-glycan signals S1H5N4F1 (C.) and H9N2 (D.) are abundantly expressed in hESC and show variable expression in the differentiated cell types. Asterisks mark statistical significant differences between sample groups according to one-way ANOVA (p < 0.05).