Figure 6

Lectin staining of hESC colonies grown on mouse feeder cell layers. A. Maackia amurensis agglutinin (MAA) that recognizes α2,3-sialylated glycans, preferably in type 2 LacNAc, stained hESC but not feeder cell surfaces. B. Pisum sativum agglutinin (PSA) that recognizes α-mannosylated glycans and core fucosylated N-glycans, stained only mouse feeder cell surfaces. C. Ulex europaeus agglutinin I (UEA-I) that recognizes α1,2-fucosylated glycans preferably within H type 2, stained the hESC colony. Mouse fibroblasts had complementary staining patterns with the lectins, indicating that their surface glycans are clearly different from hESC. D. Fluorescence-assisted cell sorting (FACS) diagrams of UEA-I selected hESC, showing that the majority of hESC were positive for cell surface UEA-I ligands.