Figure 5

Bub2 overexpression causes a defect in cell wall formation. Wild type cells with GAL1-BUB2 (KSY 129) were grown in YPGR for eight (A. and C.) or fifteen (B. and C.) hours. A. and B. FM4-64 staining to visualize the plasma membrane. Arrow points to closed bud necks. Arrowhead points to abnormal plasma membrane at the bud neck. C. Calcofluor staining shows lack of septum between buds in chains. D. Calcofluor staining shows abnormal septum formation after longer incubation in YPGR (arrowhead). Images are a single plane from a Z series and represent open or closed bud necks as determined from examination of all planes of the series. Scale bar = 5 μm.