Figure 5

Differences in the embryoid body (EB) growth rates between the hESC lines I3, I6 and BG01V. hESC colonies were removed from feeder monolayers and grown in low attachment dishes. (A) Phase contrast images of the EBs in the three cell lines cultured in suspension for 10 days. (B) A plot showing differences in the EB growth rate between three cell lines. The growth rate of EBs was calculated by the increase of EB size (total EB area) in each 5 days from day 1 though 25. Values are expressed as percent increase in total areas of EBs (mean ± SEM). Statistical differences for EB growth rates after 10 days in culture between I3 and I6 or BG01V are significant * p < 0.05. Bar in upper panel = 200 μm.