Figure 6

Differences in expression of three germ layer markers in the embryoid bodies (EBs) derived from three hES cell lines BG01V, I3 and I6. (A) Quantitative RT-PCR analysis of expression of keratin C (ectoderm marker) and alpha-Globin (mesoderm marker) at 7 and 14 days in the EBs derived from the three cell lines. The Y-axis plots the fold changes (log scale) in expression levels of the two genes for each cell line when compared to its own undifferentiated levels at Day 0. The I3-derived EBs cultured at days 7 and 14 expressed markedly higher levels of the two genes compared to I6 and BG01V-derived EBs. (B and C) Higher expression of endoderm marker AFP and mesoderm marker IGF-2 gene expression in the I3 than in the BG01V and I6 cell lines revealed by MPSS. Quantitative MPSS analysis of the AFP and IGF-2 in the EBs derived from the hES cell lines BG01V, I3 and I6. The Y-axis plots the fold change (log scale) in expression levels for each cell line when compared to its own undifferentiated cells (Day 0).