Figure 8

The comparison of the neural progenitor derivation from the I3, I6 and BG01V hESC lines over time under the same neural differentiation-promoting condition. (A) The protocol used to direct hESCs into neural cell lineages. (B-E) Phase contrast images show the progression of the neural induction of hESCs in which the hESC colonies (B) were removed from the MEF feeders and grown into floating aggregates or embryoid bodies (EBs) (C). Neural rosettes (D) were induced by the neural differentiation medium. New cells constantly generated and migrated radially away from the center of the EB and formed a rim of cells (E). Bars in B-E = 100 μm. (F-H) Neural progenitors derived from the I3 cell line. Phase contrast image of I3-differentiated cells (F) were immunostained for nestin (H) and nuclear counterstained with DAPI (G). (I) The plot shows significant differences in the average percent increase in the number of nestin+ cells differentiated from the three cell lines. Values are expressed as percent increase in Nestin+ neural progenitors (mean ± SEM). Statistical analysis shows that the percent increase in number of Nestin+ cells generated from the I3 or I6 cells is significantly greater than that generated from BG01V cells, * p < 0.05. Bar in upper panel = 200 μm.