Figure 9

Difference in directed neural differentiation between hESC lines I3, I6 and BG01V. Two days after transferring EBs to a poly-D-lysine/laminin substrate, parallel immunocytochemistry and quantitative RT-PCR were performed in hESC-derived cell populations. Immunofluorescent staining for Nestin (A-F) shows that both the I3 and the I6 cells differentiate into the enriched Nestin⁺ neural progenitors while BG01V cells barely generate Nestin⁺ cells. Bars in A-C = 100 μm. (G) qRT-PCR analysis of the gene expression of the neural progenitor markers SOX1 and MSI1, mature neuronal marker MAP2, and astrocyte marker GFAP among the three cell lines at day 17 of differentiation. The Y-axis represents the fold changes of the gene expression for each cell line when compared to its own undifferentiated levels at day 0. The relative levels of these genes expressed by the I3 and I6 cells are higher than those expressed by BG01V cells.