Subcellular-localization of TWIST full length fusion protein was monitored in transfected U2-OS cells. Transfection with empty vector (pCMV) was used as a negative control and a c-myc β-galactosidase was the positive control. Expression of c-myc-TWIST proteins was detected by fluorescence using anti-c-myc antibody. DAPI staining (blue) indicates the nucleus, while phallodin (green) represents the actin cytoskeletal staining. (b) Expression level of TWIST wild type protein shown by Western blotting with anti-c-myc antibody.