Table 3 Oligonucleotides used for mutagenesis reactions to alter NLSs sequences in TWIST.
From: Characterization of sequences in human TWIST required for nuclear localization
Amino acid substituted in NLSs | Primer Sequence |
|---|---|
K38R | 5'CGCGGGGGACGCAGGCGGCGCAGCAGC3' 5'GCTGCTGCGCCGCCTGCGTCCCCCGCG3' |
K73R | 5'CCCAGGGCAGGCGCGGCAAGAAGT3' 5'ACTTCTTGCCGCGCCTGCCCTGGG3' |
K76R | 5'CAAGCGCGGCAGGAAGTCTGCGG3' 5'CCGCAGACTTCCTGCCGCGCTTG3' |
K77R | 5'GCGGCAAGAGGTCTGCGGGCT3' 5'AGCCCGCAGACCTCTTGCCGC3' |