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Figure 8 | BMC Cell Biology

Figure 8

From: Hydrophobic profiles of the tail anchors in SLMAP dictate subcellular targeting

Figure 8

Targeting of SLMAP1-TA2 and charge. Wild type GFP-tagged SLMAP-TA2 was co-transfected into Cos7 cells with wild type Myc-tagged SLMAP-TA2 and co-stained with anti-GFP (a) and anti-6Myc antibodies (b) the overlay of the two shows that there is nearly complete overlap with a stronger GFP signal near the nucleus (c). Wild type GFP-tagged SLMAP-TA2 was co-transfected with Myc-tagged SLMAP1-TA2 K1/L and co-stained with anti-GFP (d) anti-6Myc (e) the overlay is essentially the same as the control (f). Wild type GFP-tagged SLMAP-TA2 was co-transfected with Myc-tagged SLMAP1-TA2 R27/L and co-stained with anti-GFP (g) and anti-6Myc antibodies (h) the overlay is essentially the same as the control (i). Wild type GFP-tagged SLMAP-TA2 was co-transfected with Myc-tagged SLMAP-TA2 K1/L + R27/L and co-stained with anti-GFP (j) and anti-6Myc (k) antibodies the overlay is essentially the same as the control (l).

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