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Table 2 Primers and plasmids used for this study

From: Distinctive interactions of the Arabidopsis homolog of the 30 kD subunit of the cleavage and polyadenylation specificity factor (AtCPSF30) with other polyadenylation factor subunits

 

primers

  

Designation

Sequence (5' -> 3') or description

use

Reference(s)

AtCPSF30 5'

AGATCTATGGAGGATGCTGATGGACTT

Cloning of the AtCPSF30 protein-coding region

This study

AtCPSF30 3'

CCGGAGATCTATGTCGGGCCTCCATCGATC

Cloning of the AtCPSF30 protein-coding region

This study

C-ter 30 5' (m9)

AGATCTGGAGCTGGGAGGGGTAGAAGTTTCCGTCAA

Cloning of the AtCPSF30 C-terminal protein-coding region

This study

N-ter 30 3' (m4)

GGGCCCAGGTCCAGGAAGCTTTGCATGCCTGTACCGACA

Cloning of the AtCPSF30 N-terminal protein-coding region

This study

AtDcp2 5'

CCGGAGATCTATGTCGGGCCTCCATCGATC3

Cloning of the AtDcp2 protein-coding region

This study

AtDcp2 3'

AATTGGGCCCCAAACTGACCAGTCAAGCTGAATTACCAG

Cloning of the AtDcp2 protein-coding region

This study

 

plasmids

  

designation

source

use

Reference(s)

Salk clone U61209

ABRC; corresponds to At5g13570

Template for amplification of AtDcp2 sequences

 

pMAL-AtCPSF30

Hunt laboratory

Template for amplification of AtCPSF30 sequences

[14]

pMDC43C1-GFP::AtCPSF160, pMDC43C1-GFP::AtCPSF100, pMDC43C1-GFP-AtCPSF73(I) and GFP-AtCPSF73(II)

Dr. Q. Q. Li (Miami University, Oxford, OH)

Plasmids for the expression of GFP fused Arabidopsis CPSF factors 160, 100, 73CII and 73CI

[30]

pGD RFP, pGD RFP-NLS,

Dr. Michael Goodin (University of Kentucky, Lexington, KY)

pGD RFP vector was used to express different CPSF 30 clones; pGD RFP-NLS was used as control in GFP fusion studies.

[19]

pKLX80- AtZFP11-NLS::GFP, pKLX80-CoxII::GFP, pKLX80-ER::GFP

Dinkins laboratory

 

[20, 22]