Figure 2

Ablation of PERK by AdDNPerk in 832/13 cells results in reduced insulin and proinsulin content and impaired insulin secretion. A. At 48 hr post-transduction, insulin content was assayed by ELISA in cells infected with AdDNPerk and control virus at 5, 10 and 20 MOI. The data were normalized to DNA content. B. Proinsulin content was assayed by ELISA and normalized to DNA content. C. Insulin secretion in response to 3 and 15 mmol/l glucose was measured in cells infected with recombinant virus at 5, 10 and 20 MOI. D. Insulin secretion in response to 3 and 15 mmol/l glucose with or without 200 μmol/l tolbutamide or 250 μmol/l diaxoxide. Insulin secretion in response to 15 mM glucose in C and D was normalized to total insulin content, and was expressed as a fold increases relative to insulin secreted from the control AdLacZ-832/13 cultured in 3 m mol/l glucose. Values are means and error bars are ± SE, n = 3, *p < 0.05.