Figure 5

Global protein synthesis is normal following acute ablation of PERK. A. Incorporation of 35S translabel into total TCA-precipitable protein did not differ between AdDNPerk-infected and AdLacZ-infected cells at 24 hr (A) or 16 hr post-transduction (data not shown). Values are means ± SE from three replicate samples. B. Cyclohexamide (CHX) did not inhibit the induction of ERp72 and BiP mRNA in AdDNPerk-infected 832/13 cells. At 12 hr post-transduction, cells were treated with 5 mg/ml CHX for 4 hours before RNA was extracted for Real-time quantitative RT-PCR. Values are means ± SE, from three replicate samples.