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Table 1 Temporal Changes in gene expression following Perk ablation in 832/13 cells

From: Acute ablation of PERK results in ER dysfunctions followed by reduced insulin secretion and cell proliferation

 

6 hr

16 hr

36 hr

48 hr

Ins1&II

1.02 (0.00)

1.04 (0.07)

0.64 (0.05)*

0.63 (0.12)*

MafA1

0.91 (0.10)

0.81 (0.10)

0.49 (0.12)*

0.64 (0.14)*

ERp72

0.92 (0.12)

1.81 (0.16)*

2.66 (0.52)*

3.24 (1.10)*

BiP

1.08 (0.26)

2.04 (0.32)*

4.26 (1.25)*

2.29 (0.34)*

Xbp1-S/T

1.03 (0.04)

1.35 (0.22)

1.56 (0.11)

1.32 (0.19)

Cyclin A

1.03 (0.05)

0.91 (0.06)

0.93 (0.05)

0.74 (0.10)*

Cyclin D

1.22 (0.04)

1.05 (0.08)

1.07 (0.23)

0.85 (0.11)

  1. The values represent the levels of specific mRNAs in AdDNPerk-transduced cells normalized to AdLacZ-transduced controls as determined by real-time quantitative RT-PCR. Time points (hr) indicate hours after transduction. The data are the average of two to five independent experiments. * P < 0.05
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BMC Molecular and Cell Biology

ISSN: 2661-8850

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