Figure 4

Effect of siRNA PARs on IL-12 (10 ng/ml) induced expression of PARs in P815 cells. Cells were transfected with 10 nM of siRNA PAR-1.2, siRNA PAR-2.3 or siRNA PAR-4.3 for 48 h before IL-12 being added for 6 h at 37°C. The PAR-1 (A), PAR-2 (B) and PAR-4 (C) mRNA expression was determined by real-time quantitative PCR. PAR-2 (D) and PAR-4 (E) protein expression was analyzed by flow cytometry analysis and expressed as mean fluorescence intensity. Values shown are mean ± SEM for four to five independent experiments. * P < 0.05 compared with the response of untransfected cells to the corresponding stimulus.