Figure 5

Salubrinal treatment induces apoptosis and accelerates luminal clearing in control and wild type ErbB2 acini. (A) Two representative confocal images of equatorial cross-sections of day 10 vector control, wild type and constitutively active ErbB2 acini stained for cleaved caspase-3 (c-casp-3; green) or DAPI (blue). Untreated acini (panels a, c and e) and salubrinal treated acini (panels b, d and f). Note increase in c-caspase-3 stained cells within the luminal space in salubrinal-treated vector and Wt-ErbB2 acini. Scale bars = 25 μm. (B) Graph showing distribution of the percentage of cells per acinus that stained positively for c-caspase3; mean ± SEM. Statistical significance was determined using the unpaired t-test with p < 0.05 defined as statistically significant. N.S - not significant.