Figure 3

Translocation responses of Tubby-domain and PLCδ1PH domain in AngII and ionomycin stimulated HEK293-AT1 cells. HEK293-AT1 cells were co-transfected with the GFP-Tubby-domain and the PLCδ1PH-mRFP constructs. After 24 h, cells were analyzed by confocal microscopy. Cells were stimulated with 30 nM AngII (at 16 s) followed by 10 μM ionomycin (at 117 s) as indicated. Panel A shows a representative image series recorded at the indicated time points. Panel B shows the average responses (cytoplasmic fluorescence increase) of 50-54 cells (mean ± S.E.M) (left) and the average curve normalized to the maximal cytoplasmic fluorescence attained after ionomycin treatment (full translocation) (Panel B, right). Note the significantly slower and only partial response of the Tubby domain after AngII stimulation.