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Figure 4 | BMC Cell Biology

Figure 4

From: Live cell imaging with protein domains capable of recognizing phosphatidylinositol 4,5-bisphosphate; a comparative study

Figure 4

Distribution of the Tubby-domain and PLCδ1PH domain in cells expressing a constitutively active Gq protein. HEK293-AT1 cells were co-transfected with the GFP-Tubby-domain, PLCδ1-PH-GFP plasmids together with a constitutively active Gq α-subunit (Q209L). Panel A shows cells in which the PLCδ1-PH localization is completely lost while that of the Tubby domain is still quite substantial (upper) while in some cells both construct completely lose membrane localization (lower). Panel B shows an example where a cell that still shows substantial localization of the Tubby domain loses its PM localization after stimulation with even a low concentration (10 nM) of AngII.

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