Table 1 Localization responses of selected yeast PH domains and the human Tubby domain expressed in HEK293-AT1 or COS-7 cells.
PH domain | Subcellular Localization | Response to AngII stimulation | Response to PtdIns(4,5)P2 elimination by 5-ptase | Presumptive inositide dependence of PM localization |
|---|---|---|---|---|
PLCδ1-PH | PM | transient translocation | complete translocation | PtdIns(4,5)P2 |
Cla4p-PH | PM | no change | no change | inositide independent |
Num1p-PH | PM | transient translocation | complete translocation | PtdIns(4,5)P2 |
Skm1p-PH | PM | no change | no change | inositide independent |
Opy1p-PH | PM | transient translocation | complete translocation | PtdIns(4,5)P2 |
Yil105c/ Slm1p-PH | PM | transient translocation | partial translocation | PtdIns(4,5)P2 PtdIns4P |
Ynl047/ Slm2p-PH | PM | no change | no change | inositide independent |
Tubby domain | PM | transient translocation | complete translocation | PtdIns(4,5)P2 |