Table 1 Deleted MAL2 yeast two-hybrid constructs, primer sequences and MAL2 regions deleted
From: Mucin 1 (MUC1) is a novel partner for MAL2 in breast carcinoma cells
| Construct | PCR Primer Sequences (5'-3') | MAL2 region deleted |
|---|---|---|
|
pAS2-1MAL2ΔN pACT2MAL2ΔN |
CATGCCATGGCCTACTCGGGCGCCTTCGTC CCGCTCGAGTTACGGTCGCCATCTTCGTAA |
amino acids 1–34 |
|
pAS2-1MAL2 ΔNTM1 |
CCGGAATTCTCCTCCAATGTTCCTCTACC CCGCTCGAGTTACGGTCGCCATCTTCGTAA |
amino acids 1–55 |
|
pAS2-1MAL2 ΔNTM12 |
CCGGAATTCCTCTCTGGAATGGTGGCT CCGCTCGAGTTACGGTCGCCATCTTCGTAA |
amino acids 1–86 |
|
pAS2-1MAL2 ΔNTM123 |
CCGGAATTCGAAGCAGCAGCCACATCC CCGCTCGAGTTACGGTCGCCATCTTCGTAA |
amino acids 1–119 |
|
pAS2-1MAL2 ΔNTM1234 |
CCGGAATTCGCTTTACGAAGATGGCGACCG CCGCTCGAGTTACGGTCGCCATCTTCGTAA |
amino acids 1–169 |