Table 1 Deleted MAL2 yeast two-hybrid constructs, primer sequences and MAL2 regions deleted
From: Mucin 1 (MUC1) is a novel partner for MAL2 in breast carcinoma cells
Construct | PCR Primer Sequences (5'-3') | MAL2 region deleted |
|---|---|---|
pAS2-1MAL2ΔN pACT2MAL2ΔN | CATGCCATGGCCTACTCGGGCGCCTTCGTC CCGCTCGAGTTACGGTCGCCATCTTCGTAA | amino acids 1–34 |
pAS2-1MAL2 ΔNTM1 | CCGGAATTCTCCTCCAATGTTCCTCTACC CCGCTCGAGTTACGGTCGCCATCTTCGTAA | amino acids 1–55 |
pAS2-1MAL2 ΔNTM12 | CCGGAATTCCTCTCTGGAATGGTGGCT CCGCTCGAGTTACGGTCGCCATCTTCGTAA | amino acids 1–86 |
pAS2-1MAL2 ΔNTM123 | CCGGAATTCGAAGCAGCAGCCACATCC CCGCTCGAGTTACGGTCGCCATCTTCGTAA | amino acids 1–119 |
pAS2-1MAL2 ΔNTM1234 | CCGGAATTCGCTTTACGAAGATGGCGACCG CCGCTCGAGTTACGGTCGCCATCTTCGTAA | amino acids 1–169 |