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Figure 2 | BMC Cell Biology

Figure 2

From: Defects in cellular sorting and retroviral assembly induced by GGA overexpression

Figure 2

Treatment with BFA dissociates endogenous GGAs from membranes but does not disrupt the structures induced by GGA overexpression. HeLa cells were left untransfected, or were transfected with 0.5 μg vectors expressing WT GGAs or NA mutants defective in Arf binding (NA). Approximately 24 h posttransfection, cells were left untreated (-BFA) or were treated with BFA at 4 μg/ml for 1 h (+BFA). Cells were then fixed, stained with anti-GGA antibodies for visualization of endogenous GGAs or anti-Myc antibody for detection of exogenous GGAs, and analyzed by fluorescence microscopy. Size bar represents 15 μm.

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