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Figure 3 | BMC Cell Biology

Figure 3

From: Defects in cellular sorting and retroviral assembly induced by GGA overexpression

Figure 3

GGA-induced compartments colocalize with the aggresome marker GFP-250. (A) HeLa cells were cotransfected with vectors expressing Myc-tagged GGAs or HA-tagged TSG-3' and GFP-250 at a 1:1 input DNA ratio. Cells were fixed approximately 24 h posttransfection, stained with anti-Myc or anti-HA antibodies, and analyzed by fluorescence microscopy. Numbers represent mean colocalization (R value) ± SD from 10 cells. Size bar represents 15 μm. (B) HeLa cells transfected with GGA or TSG-3' expression vectors were fixed 24 h posttransfection and analyzed by transmission EM. Size bar represents 500 nm.

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