Figure 5
From: Defects in cellular sorting and retroviral assembly induced by GGA overexpression
GGA overexpression alters the localization of endosomal sorting factors. HeLa cells were transfected with vectors expressing (A) Myc-tagged GGAs and HA-tagged TSG-F or (B) GFP-tagged GGAs and Myc-tagged Hrs. Cells were fixed approximately 24 h posttransfection, stained with anti-HA and anti-Myc antibodies (A) or anti-Myc antibody (B) followed by microscopy. Numbers represent mean colocalization (R value) ± SD from 8-10 cells. Size bar represents 15 μm.