Figure 7
From: Defects in cellular sorting and retroviral assembly induced by GGA overexpression
Sequestration of retroviral Gag proteins in GGA1-induced structures. (A) HeLa cells were transfected with the Myc-tagged GGA1 expression vector along with WT pNL4-3, or the Fyn10fullMA, Fyn10deltaMA, or 1GA derivatives. Cells were fixed approximately 24 h posttransfection, stained using anti-Myc and anti-HIV-1 p24 antibodies followed by immunofluorescence analysis. Numbers represent average colocalization (R value) ± SD from 10 cells. Size bar represents 15 μm. (B) HeLa cells were cotransfected with vectors expressing Myc-tagged GGA1 and EIAV Gag. Cells were fixed, stained using anti-Myc and anti-EIAV p26 antibodies and analyzed as indicated above. Numbers represent mean colocalization (R value) ± SD from 7-10 cells.