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Figure 4 | BMC Cell Biology

Figure 4

From: Inhibition of extracellular matrix assembly induces the expression of osteogenic markers in skeletal muscle cells by a BMP-2 independent mechanism

Figure 4

Cbfa-1 expression is not induced by inhibition of proteoglycan sulfation. RNA from C2C12 cells after 6 days of differentiation in the absence or presence of 1 nM BMP-2 or 30 mM sodium chlorate was obtained. After reverse transcription using Oligo-dTs, Cbfa-1, myogenin and β-actin cDNAs were amplified using specific primers. The reactions were performed within the linear range for both time and RNA quantity.

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