Figure 5
Alkaline phosphatase activity induced by inhibition of proteoglycan sulfation is not BMP-2 dependent. A. C2C12 cells were induced to differentiate in the presence of 5 nM BMP-2. ALP (open circles) and CK (closed circles) activity was determined as a function of time. The mean ± S.D. of two independent experiments performed in triplicate is presented. B. C2C12 cells were induced to differentiate in the presence of 5 nM BMP-2 and different concentrations of soluble BMP receptor IA (BMPR-IAsoluble). ALP (open circles) and CK (closed circles) activity was determined at day 6. Values presented as mean ± S.D. of two independent experiments performed in triplicate. C2C12 cells were induced to differentiate either in the absence (control) or presence of 30 mM sodium chlorate (chlorate) or 1 μg/ml soluble BMP receptor IA (BMPR-IAsoluble). ALP (C) and CK (D) activity was determined at day 8 days. The mean ± S.E.M, of two independent experiments performed in triplicate is presented. ANOVA analysis followed by Tukey-Kramer multiple comparisons test shows that there are no statistically significant differences (p > 0.05) within the control or chlorate conditions. All other comparisons are significantly different (p < 0.001).